Trans-Generational Symbiont Transmission Reduced at High Temperatures in a West Nile Virus Vector Mosquito Culex quinquefasciatus

The influence of environmental factors on the efficacy endosymbiont Wolbachia used in mosquito and pathogen control are poorly characterized may be critical for disease control. We studied vector Culex pipiens quinquefasciatus (Say) to determine effect temperature composition relative abundance spp. microbiome, as well key immune genes interest Toll IMD pathways. 16S barcode sequencing was microbiome qPCR based highly utilized marker surface protein ( wsp ) gene. found no within a single generation or gene expression, nor alpha beta diversity microbiome. However, there significant difference between generations at high temperatures (≥ 28°C), but not lower (≤ 23°C). These results support idea that reduced higher generations, which has an establishment pathogens including West Nile Virus (WNV). Modulation pathways indicated. endosymbiosis trans-generation transmission appears especially sensitive temperatures, have implications Wolbachia- strategies under climate change scenarios.